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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

9,823 Views

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08:40 min

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November 29, 2016

DOI:

10.3791/54901-v

DOI:
10.3791/54901-v

•

08:40 min
November 29, 2016

•

11 Views
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1Program in Developmental Biology,Baylor College of Medicine, 2Department of Genetics,UT MD Anderson Cancer Center

Summary

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We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

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Cite this Article

Gervaise, A. L., Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (117), e54901, doi:10.3791/54901 (2016).

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