Journal
/
Developmental Biology
/
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

English

Automatically Generated

9,823 Views

•

08:40 min

•

November 29, 2016

DOI:

10.3791/54901-v

DOI:
10.3791/54901-v

•

08:40 min
November 29, 2016

•

11 Views
,
1Program in Developmental Biology,Baylor College of Medicine, 2Department of Genetics,UT MD Anderson Cancer Center

Summary

Automatically generated

We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

Explore More Videos

ERK SignalingC. ElegansGermlineIn VivoQuantificationSpatial And Temporal AnalysisDrug TreatmentsGene PerturbationsDissectionImmunofluorescenceMicroscopy

Read Article

Cite this Article

Gervaise, A. L., Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (117), e54901, doi:10.3791/54901 (2016).

Download .ris file

Copy

Share Video

.

Copy