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Journal
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Immunology and Infection
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抗体标记用荧光染料使用磁蛋白A和G蛋白珠
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
Please note that all translations are automatically generated.
Click here for the English version.
抗体标记用荧光染料使用磁蛋白A和G蛋白珠
DOI:
10.3791/54545-v
•
06:48 min
•
September 15, 2016
•
Nidhi Nath
,
Becky Godat
,
Marjeta Urh
1
Promega Corporation
Chapters
00:05
Title
00:41
High Capacity Magnetic Protein A or G Bead Antibody Capture
01:43
Antibody Labeling Using Amine Chemistry
02:56
Antibody Labeling Using Thiol Chemistry
04:46
Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
05:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
上的胎圈的方法用于标记抗体与小分子使抗体的少量的标记直接从细胞培养基。这种方法是与胺和硫醇化学相容,并且可以并行处理多个样品,手工或使用自动平台。
Tags
Antibody Labeling
Fluorescent Dyes
Magnetic Protein A Beads
Protein G Beads
Amine Chemistry
Thiol Chemistry
Antibody Purification
Cell Medium
Automated Labeling
Manual Labeling
Absorbance Measurement
Conjugation Buffer
Elution Buffer
Neutralization Buffer
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