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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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JoVE 杂志 免疫与感染
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

DOI:
10.3791/54545-v

•

06:48 min

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September 15, 2016

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, ,
1Promega Corporation

Chapters

  • 00:05Title
  • 00:41High Capacity Magnetic Protein A or G Bead Antibody Capture
  • 01:43Antibody Labeling Using Amine Chemistry
  • 02:56Antibody Labeling Using Thiol Chemistry
  • 04:46Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
  • 05:39Conclusion

Summary

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The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.

Tags

Antibody LabelingFluorescent DyesMagnetic Protein A BeadsProtein G BeadsAmine ChemistryThiol ChemistryAntibody PurificationCell MediumAutomated LabelingManual LabelingAbsorbance MeasurementConjugation BufferElution BufferNeutralization Buffer

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