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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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September 25, 2011
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We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
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Colorectal CancerCell Surface Protein ProfilingAntibody MicroarrayFluorescence MultiplexingCRC PrognosisProtein ExpressionPost-translational ModificationCluster Of Differentiation AntigensBiomarkersImmunohistochemistry (IHC)Cancer Sub-classificationPrognosticationMarkerClinico-pathological StagingProteomic TechniquesITRAQDiagnostic Laboratories
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Zhou, J., Belov, L., Solomon, M. J., Chan, C., Clarke, S. J., Christopherson, R. I. Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing. J. Vis. Exp. (55), e3322, doi:10.3791/3322 (2011).
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