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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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September 15, 2016
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Summary
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The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
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Nath, N., Godat, B., Urh, M. Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads. J. Vis. Exp. (115), e54545, doi:10.3791/54545 (2016).
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