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1Innovation Laboratory, Center for Molecular Oncology,Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility,Weill Cornell Medical College, 3Department of Laboratory Medicine,University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine,Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine,Weill Cornell Medical College, 6Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center
Chapters
- 00:05Title
- 01:19Cell Suspension Preparation for Sorting
- 03:00Compensation Control Setup
- 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
- 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
- 08:02Conclusion
Aqui, descrevemos uma classificação de células de citometria de fluxo combinado e um protocolo de construção de bibliotecas de próxima geração, de baixa entrada, projetado para produzir dados de alta qualidade e total de exomas das células Hodgkin Reed-Sternberg (HRS) do linfoma de Hodgkin clássico (CHL).
