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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
JoVE Journal
Genetics
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JoVE Journal Genetics
Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

English

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13,036 Views

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11:26 min

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June 03, 2019

DOI:

10.3791/59377-v

DOI:
10.3791/59377-v

•

11:26 min
June 03, 2019

•

11 Views
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1Institute of Molecular Virology and Cell Biology,Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases,Friedrich-Loeffler-Institut, 3Department of Animal Science,Njala University

Summary

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Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

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Cite this Article

Mueller, A., Fischer, K., Suluku, R., Hoenen, T. Sequencing of mRNA from Whole Blood using Nanopore Sequencing. J. Vis. Exp. (148), e59377, doi:10.3791/59377 (2019).

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