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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
JoVE Journal
Genetics
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JoVE Journal Genetics
Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

DOI:
10.3791/59377-v

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11:26 min

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June 03, 2019

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, , ,
1Institute of Molecular Virology and Cell Biology,Friedrich-Loeffler-Institut, 2Institute of Novel and Emerging Infectious Diseases,Friedrich-Loeffler-Institut, 3Department of Animal Science,Njala University

Chapters

  • 00:04Title
  • 00:54Amplification of the NPC1 Open Reading Frame
  • 03:36Library Preparation
  • 05:48Quality Check of Flow Cell
  • 06:31Loading the Flow Cell and Starting the Sequencing Run
  • 08:59Results: Analysis of Sequenced mRNA
  • 10:30Conclusion

Summary

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Nanopore sequencing is a novel technology that allows cost-effective sequencing in remote locations and resource-poor settings. Here, we present a protocol for sequencing of mRNAs from whole blood that is compatible with such conditions.

Tags

MRNA SequencingNanopore SequencingThird-generation SequencingField LaboratoriesEbola VirusTouchdown PCRCDNA AmplificationMagnetic Bead PurificationEthanol WashLibrary Preparation

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