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Genetics

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JoVE Journal Genetics
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
 

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

Article DOI: 10.3791/54399-v • 08:53 min • June 10th, 2017
June 10th, 2017 •
1, 2, 3, 4, 5, 6
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center

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Here, we describe a combined flow cytometric cell sorting and low-input, next-generation library construction protocol designed to produce high-quality, whole-exome data from the Hodgkin Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (CHL).

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Flow-sorting Exome Sequencing Reed-Sternberg Cells Classical Hodgkin Lymphoma HRS Cells CHL Tumors Next Generation Sequencing Data Key Driver Mutations Immuno Escape Mutations Genic Diversity Tumor Derived DNA High Quality And Purity Next Generation Sequencing Libraries Diagnosis Of Hodgkin Lymphoma Sub Classification Prognostication Therapy Choice Flow Cytometry Sorting Experiments
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