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1Innovation Laboratory, Center for Molecular Oncology,Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility,Weill Cornell Medical College, 3Department of Laboratory Medicine,University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine,Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine,Weill Cornell Medical College, 6Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center
Chapters
- 00:05Title
- 01:19Cell Suspension Preparation for Sorting
- 03:00Compensation Control Setup
- 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
- 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
- 08:02Conclusion
Qui descriviamo un sistema di classificazione delle celle citometriche a flusso combinato e un protocollo di costruzione della libreria di nuova generazione, progettato per produrre dati di alta qualità e di esito intero dalle cellule Hodgkin Reed-Sternberg (HRS) del linfoma classico Hodgkin (CHL).
