Journal
/
Genetics
/
Detection of Copy Number Alterations Using Single Cell Sequencing
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

DOI:
10.3791/55143-v

•

09:45 min

•

February 17, 2017

•

, ,
1Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, 2Howard Hughes Medical Institute, 3Division of Health Sciences and Technology,Harvard Medical School, 4The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 5BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Chapters

  • 00:05Title
  • 00:48Preparation of Aspirator
  • 04:34Whole Genome Amplification
  • 07:39Results: Detection of Copy Number Alterations Using Single Cell Sequencing
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

Single cell sequencing is an increasingly popular and accessible tool for addressing genomic changes at high resolution. We provide a protocol that uses single cell sequencing to identify copy number alterations in single cells.

Tags

Single Cell SequencingCopy Number AlterationsGenomic HeterogeneityCytologic ApproachesSensitivitySpecificityMicro-aspiratorSyringe FilterPVC TubingSerological PipetteGlass Capillary TubeWhole Genome AmplificationAdherence CellsTrypsinization10% Bleach70% Ethanol

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Identification of Circular RNAs using RNA Sequencing

Read Article