A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Innovation Laboratory, Center for Molecular Oncology,Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility,Weill Cornell Medical College, 3Department of Laboratory Medicine,University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine,Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine,Weill Cornell Medical College, 6Department of Laboratory Medicine,Memorial Sloan Kettering Cancer Center
Chapters
- 00:05Title
- 01:19Cell Suspension Preparation for Sorting
- 03:00Compensation Control Setup
- 04:53Hodgkin Reed-Sternberg-, B-, and T-cell Isolation
- 06:07Results: Representative DNA Library and Copy Number Gains and Losses Analyses
- 08:02Conclusion
Hier beschrijven we een gecombineerde flow cytometrische celsortering en een laag ingevoerde, volgende generatie bibliotheekconstructieprotocol dat is ontworpen om hoogwaardige, volledige exome data van de Hodgkin Reed-Sternberg (HRS) cellen van klassiek Hodgkin lymfoom (CHL) te produceren.
