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为患者组织甲基结合DNA序列捕获

JoVE Journal
Genetics

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JoVE Journal Genetics

Methyl-binding DNA capture Sequencing for Patient Tissues

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为患者组织甲基结合DNA序列捕获

DOI:

10.3791/54131-v

•

08:40 min

•

October 31, 2016

•

Rohit R. Jadhav, Yao V. Wang, Ya-Ting Hsu, Joseph Liu, Dawn Garcia, Zhao Lai, Tim H. M. Huang, Victor X. Jin

¹Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, ²Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

Chapters

00:05Title
01:18Methyl-binding DNA Capture
02:10Single Fraction Elution
03:08Ethanol Precipitation
04:29Sequencing
05:58Results: Sequencing of Methyl-binding DNA Capture
07:15Conclusion

Summary

Automatic Translation

在这里，我们提出了一个协议，调查使用甲基结合DNA测序捕获（MBDCap-SEQ或MBD-SEQ）技术以及随后的生物信息学分析管道的大规模临床病人的筛查研究全基因组DNA甲基化。

Tags

DNA Methylation Epigenetics Patient Cohorts Biomarkers Methyl-binding DNA Capture Sequencing Genome-wide Analysis Cost-effective Sonication Fragment Size Elution Magnetic Beads

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