Journal
/
Genetics
/
Metil-binding cattura sequenziamento del DNA per tessuti del paziente
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Methyl-binding DNA capture Sequencing for Patient Tissues
Please note that all translations are automatically generated. Click here for the English version.

Metil-binding cattura sequenziamento del DNA per tessuti del paziente

italiano

Automatically Generated

8,424 Views

•

08:40 min

•

October 31, 2016

DOI:

10.3791/54131-v

DOI:
10.3791/54131-v

•

08:40 min
October 31, 2016

•

14 Views
, , , , , , ,
1Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, 2Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

Summary

Automatically generated

Qui vi presentiamo un protocollo per indagare genoma metilazione del DNA in studi clinici di screening dei pazienti su larga scala utilizzando il metil-Binding sequenziamento del DNA di cattura (MBDCap-ss o MBD-ss), la tecnologia e la successiva analisi bioinformatica pipeline.

Explore More Videos

DNA MethylationEpigeneticsPatient CohortsBiomarkersMethyl-binding DNA CaptureSequencingGenome-wide AnalysisCost-effectiveSonicationFragment SizeElutionMagnetic Beads

Read Article

Cite this Article

Jadhav, R. R., Wang, Y. V., Hsu, Y., Liu, J., Garcia, D., Lai, Z., Huang, T. H. M., Jin, V. X. Methyl-binding DNA capture Sequencing for Patient Tissues. J. Vis. Exp. (116), e54131, doi:10.3791/54131 (2016).

Download .ris file

Copy

Share Video

.

Copy