Journal
/
Genetics
/
Secuenciación de captura de ADN de unión a metil-para tejidos del paciente
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Methyl-binding DNA capture Sequencing for Patient Tissues
Please note that all translations are automatically generated. Click here for the English version.

Secuenciación de captura de ADN de unión a metil-para tejidos del paciente

español

Automatically Generated

8,424 Views

•

08:40 min

•

October 31, 2016

DOI:

10.3791/54131-v

DOI:
10.3791/54131-v

•

08:40 min
October 31, 2016

•

14 Views
, , , , , , ,
1Department of Molecular Medicine,University of Texas Health Science Center at San Antonio, 2Greehey Children’s Cancer Research Institute,University of Texas Health Science Center at San Antonio

Summary

Automatically generated

Aquí se presenta un protocolo para investigar la metilación del ADN del genoma de ancho en estudios de cribado de pacientes clínicos a gran escala utilizando la secuenciación Metil-unión de captura de ADN (SEC-MBDCap o MBD-ss) la tecnología y el análisis de tuberías bioinformática posteriores.

Explore More Videos

DNA MethylationEpigeneticsPatient CohortsBiomarkersMethyl-binding DNA CaptureSequencingGenome-wide AnalysisCost-effectiveSonicationFragment SizeElutionMagnetic Beads

Read Article

Cite this Article

Jadhav, R. R., Wang, Y. V., Hsu, Y., Liu, J., Garcia, D., Lai, Z., Huang, T. H. M., Jin, V. X. Methyl-binding DNA capture Sequencing for Patient Tissues. J. Vis. Exp. (116), e54131, doi:10.3791/54131 (2016).

Download .ris file

Copy

Share Video

.

Copy