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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
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September 11, 2016
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Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.
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EPA Method 1615Virus MeasurementTotal Culturable Virus AssayAlkaline-based Extract SolutionElectro-positive FilterVirus ElutionVirus ConcentrationVirus FlocculationVirus IsolationBuffalo Green Monkey Kidney CellsCytopathic Effect (CPE)Most Probable Number (MPN)EnterovirusNorovirusDrinking WaterEnvironmental WaterWater QualityVirus DetectionVirus Quantification
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Fout, G. S., Cashdollar, J. L. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay. J. Vis. Exp. (115), e52437, doi:10.3791/52437 (2016).
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