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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
JoVE Revista
Medio ambiente
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JoVE Revista Medio ambiente
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

DOI:
10.3791/52437-v

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11:09 min

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September 11, 2016

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,
1National Exposure Research Laboratory,U.S. Environmental Protection Agency

Capítulos

  • 00:05Título
  • 02:32Filter Elution Procedure
  • 03:33Organic Flocculation Concentration Procedure
  • 06:03Total Culturable Virus Quantal Assay
  • 09:26Results: Mean Poliovirus Recovery (%) from Ground and Reagent-grade Water
  • 10:19Conclusion

Summary

Traducción Automática

Traducción Automática

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

Tags

Keywords: EPA Method 1615Virus MeasurementTotal Culturable Virus AssayAlkaline-based Extract SolutionElectro-positive FilterVirus ElutionVirus ConcentrationVirus FlocculationVirus IsolationBuffalo Green Monkey Kidney CellsCytopathic Effect (CPE)Most Probable Number (MPN)EnterovirusNorovirusDrinking WaterEnvironmental WaterWater QualityVirus DetectionVirus Quantification

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