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Journal / Environment / EPA Method 1615. Measurement of Enterovirus and…
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JoVE Journal Environment
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
 

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay

Article DOI: 10.3791/52437-v • 11:09 min • September 11th, 2016
September 11th, 2016 •
1, 1
1National Exposure Research Laboratory, U.S. Environmental Protection Agency

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Summary
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Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

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Keywords: EPA Method 1615 Virus Measurement Total Culturable Virus Assay Alkaline-based Extract Solution Electro-positive Filter Virus Elution Virus Concentration Virus Flocculation Virus Isolation Buffalo Green Monkey Kidney Cells Cytopathic Effect (CPE) Most Probable Number (MPN) Enterovirus Norovirus Drinking Water Environmental Water Water Quality Virus Detection Virus Quantification
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