Journal
/
Environment
/
エンテロウイルスと文化とRT-qPCRにより水中のノロウイルス発生のEPAメソッド1615計測。 II。総培養可能なウイルスアッセイ
JoVE Journal
Environment
This content is Free Access.
JoVE Journal Environment
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
Please note that all translations are automatically generated. Click here for the English version.

エンテロウイルスと文化とRT-qPCRにより水中のノロウイルス発生のEPAメソッド1615計測。 II。総培養可能なウイルスアッセイ

日本語

Automatically Generated

8,485 Views

•

11:09 min

•

September 11, 2016

DOI:

10.3791/52437-v

DOI:
10.3791/52437-v

•

11:09 min
September 11, 2016

•

4 Views
,
1National Exposure Research Laboratory,U.S. Environmental Protection Agency

Summary

Automatically generated

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

Explore More Videos

EPA Method 1615Virus MeasurementTotal Culturable Virus AssayAlkaline-based Extract SolutionElectro-positive FilterVirus ElutionVirus ConcentrationVirus FlocculationVirus IsolationBuffalo Green Monkey Kidney CellsCytopathic Effect (CPE)Most Probable Number (MPN)EnterovirusNorovirusDrinking WaterEnvironmental WaterWater QualityVirus DetectionVirus Quantification

Read Article

Cite this Article

Fout, G. S., Cashdollar, J. L. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay. J. Vis. Exp. (115), e52437, doi:10.3791/52437 (2016).

Download .ris file

Copy

Share Video

.

Copy