This content is Free Access.
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
8,485 Views
•
11:09 min
•
September 11, 2016
DOI:
Summary
Automatically generated
Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.
Explore More Videos
EPA Method 1615Virus MeasurementTotal Culturable Virus AssayAlkaline-based Extract SolutionElectro-positive FilterVirus ElutionVirus ConcentrationVirus FlocculationVirus IsolationBuffalo Green Monkey Kidney CellsCytopathic Effect (CPE)Most Probable Number (MPN)EnterovirusNorovirusDrinking WaterEnvironmental WaterWater QualityVirus DetectionVirus Quantification
Read Article
Cite this Article
Fout, G. S., Cashdollar, J. L. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay. J. Vis. Exp. (115), e52437, doi:10.3791/52437 (2016).
Copy