/
/
Antigener Beskyttet Funksjonell røde blodceller av membranen Pode av kompakt hyperforgrenede Polyglycerols
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:28Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
- 03:30Whole Blood Collection and Separation of Red Blood Cells (RBC)
- 04:26HPG Grafting to RBC
- 05:28Characterization of HPG Modified RBC: Complement Mediated Lysis
- 07:16Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
- 08:08Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
- 09:14Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
- 10:12Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
- 10:58Conclusion
Cellemembranen modifisering av røde blodceller (RBC) med hyperforgrenede polyglycerol (HPG) presenteres. Modifiserte RBCs var preget av vandig to fase partisjonering, osmotisk skjørhet og utfyller mediert lysis. Kamuflasje av overflateproteiner og antigener ble evaluert med flowcytometri og Micro Typing System (MTS) blod fenotyping kort.
