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Gli antigeni Protetto funzionali globuli rossi dalla membrana innesto di Compact Poligliceroli Hyperbranched
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
DOI:

11:31 min

January 02, 2013

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Chapters

  • 00:05Title
  • 01:28Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
  • 03:30Whole Blood Collection and Separation of Red Blood Cells (RBC)
  • 04:26HPG Grafting to RBC
  • 05:28Characterization of HPG Modified RBC: Complement Mediated Lysis
  • 07:16Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
  • 08:08Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
  • 09:14Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
  • 10:12Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
  • 10:58Conclusion

Summary

Automatic Translation

La modifica membrana cellulare dei globuli rossi (RBC) con poliglicerolo hyperbranched (HPG) è presentato. RBC modificati sono stati caratterizzati da due partizionamento fase acquosa, la fragilità osmotica e lisi complemento mediata. Il camuffamento di proteine ​​di superficie e degli antigeni è stata valutata utilizzando la citometria a flusso e sistema Micro Typing (MTS) carte fenotipizzazione sangue.

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