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Gli antigeni Protetto funzionali globuli rossi dalla membrana innesto di Compact Poligliceroli Hyperbranched
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Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Gli antigeni Protetto funzionali globuli rossi dalla membrana innesto di Compact Poligliceroli Hyperbranched

italiano

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17,925 Views

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11:31 min

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January 02, 2013

DOI:

10.3791/50075-v

DOI:
10.3791/50075-v

•

11:31 min
January 02, 2013

•

1 Views
, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Summary

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La modifica membrana cellulare dei globuli rossi (RBC) con poliglicerolo hyperbranched (HPG) è presentato. RBC modificati sono stati caratterizzati da due partizionamento fase acquosa, la fragilità osmotica e lisi complemento mediata. Il camuffamento di proteine ​​di superficie e degli antigeni è stata valutata utilizzando la citometria a flusso e sistema Micro Typing (MTS) carte fenotipizzazione sangue.

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Cite this Article

Chapanian, R., Constantinescu, I., Brooks, D. E., Scott, M. D., Kizhakkedathu, J. Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols. J. Vis. Exp. (71), e50075, doi:10.3791/50075 (2013).

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