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紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞
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Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞

English

Automatically Generated

17,922 Views

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11:31 min

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January 02, 2013

DOI:

10.3791/50075-v

DOI:
10.3791/50075-v

•

11:31 min
January 02, 2013

•

1 Views
, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Summary

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细胞膜的变形例的红血细胞（红细胞），超支化聚甘油（HPG）。修改红细胞进行了表征双水相分割，渗透脆性和补体介导的溶解。使用流式细胞仪和微的打字系统（MTS）的血液分型卡的表面蛋白抗原的伪装进行了评估。

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Cite this Article

Chapanian, R., Constantinescu, I., Brooks, D. E., Scott, M. D., Kizhakkedathu, J. Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols. J. Vis. Exp. (71), e50075, doi:10.3791/50075 (2013).

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