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Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

English

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17,925 Views

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11:31 min

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January 02, 2013

DOI:

10.3791/50075-v

DOI:
10.3791/50075-v

•

11:31 min
January 02, 2013

•

1 Views
, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Summary

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The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.

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Cite this Article

Chapanian, R., Constantinescu, I., Brooks, D. E., Scott, M. D., Kizhakkedathu, J. Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols. J. Vis. Exp. (71), e50075, doi:10.3791/50075 (2013).

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