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Antigenen functionele rode bloedcellen die beschermd worden door de membraan enten van Compact Hypervertakte Polyglycerolen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Antigenen functionele rode bloedcellen die beschermd worden door de membraan enten van Compact Hypervertakte Polyglycerolen

Nederlands

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17,922 Views

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11:31 min

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January 02, 2013

DOI:

10.3791/50075-v

DOI:
10.3791/50075-v

•

11:31 min
January 02, 2013

•

1 Views
, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Summary

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Het celmembraan modificatie van rode bloedcellen (RBC's) met hypervertakte polyglycerol (HPG) wordt getoond. Gewijzigd RBC werden gekenmerkt door waterige twee fase partitionering, osmotische fragiliteit en complement gemedieerde lysis. De camouflage van de oppervlakte-eiwitten en antigenen werd geëvalueerd met behulp van de flowcytometrie en Micro Typing System (MTS) bloed fenotypering kaarten.

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Cite this Article

Chapanian, R., Constantinescu, I., Brooks, D. E., Scott, M. D., Kizhakkedathu, J. Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols. J. Vis. Exp. (71), e50075, doi:10.3791/50075 (2013).

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