Journal
/
면역학 및 감염병학
/
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

17,925 Views

•

11:31 min

•

January 02, 2013

DOI:

10.3791/50075-v

DOI:
10.3791/50075-v

•

11:31 min
January 02, 2013

•

1 Views
, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Summary

Automatically generated

The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.

Explore More Videos

Antigen ProtectionRed Blood CellsMembrane GraftingHyperbranched PolyglycerolAlloantibodiesUniversal Red Blood DonorTransfusion Safety

Read Article

Cite this Article

Chapanian, R., Constantinescu, I., Brooks, D. E., Scott, M. D., Kizhakkedathu, J. Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols. J. Vis. Exp. (71), e50075, doi:10.3791/50075 (2013).

Download .ris file

Copy

Share Video

.

Copy