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항원은 컴팩트 Hyperbranched Polyglycerols 중 이식 막으로 기능 적혈구를 보호
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
DOI:

11:31 min

January 02, 2013

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Chapters

  • 00:05Title
  • 01:28Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
  • 03:30Whole Blood Collection and Separation of Red Blood Cells (RBC)
  • 04:26HPG Grafting to RBC
  • 05:28Characterization of HPG Modified RBC: Complement Mediated Lysis
  • 07:16Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
  • 08:08Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
  • 09:14Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
  • 10:12Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
  • 10:58Conclusion

Summary

Automatic Translation

hyperbranched polyglycerol (HPG)와 적혈구의 세포막 수정 (적혈구)가 제공됩니다. 수정 적혈구는 수성 2 상 분할, 삼투 취약성과 보완 매개 용해에 의해 특징되었습니다. 표면 단백질과 항원의 위장은 유동 세포 계측법 및 마이크로 타이핑 시스템 (MTS) 혈액 phenotyping 카드를 사용 평가되었습니다.

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