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Rilevamento di Rare varianti genomiche da Sequencing pool Uso SPLINTER
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Biology
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Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
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Rilevamento di Rare varianti genomiche da Sequencing pool Uso SPLINTER

italiano

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14,862 Views

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14:06 min

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June 23, 2012

DOI:

10.3791/3943-v

DOI:
10.3791/3943-v

•

14:06 min
June 23, 2012

•

3 Views
, , , ,
1Center for Genome Sciences and Systems Biology, Department of Genetics,Washington University School of Medicine, 2Department of Internal Medicine,Washington University School of Medicine, 3Department of Pediatrics,Washington University School of Medicine

Summary

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Sequenziamento del DNA pool è una strategia rapida ed efficace per individuare le varianti rare associate a fenotipi complessi in coorti di grandi dimensioni. Qui si descrive l'analisi computazionale del pool, sequenziamento di prossima generazione di 32 geni legati al cancro utilizzando il pacchetto software SPLINTER. Questo metodo è scalabile, e applicabile a qualsiasi fenotipo di interesse.

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Cite this Article

Vallania, F., Ramos, E., Cresci, S., Mitra, R. D., Druley, T. E. Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER. J. Vis. Exp. (64), e3943, doi:10.3791/3943 (2012).

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