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Biolumineszenz Imaging von NADPH-Oxidase-Aktivität in verschiedenen Tiermodellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Biolumineszenz Imaging von NADPH-Oxidase-Aktivität in verschiedenen Tiermodellen

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16,071 Views

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10:42 min

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October 22, 2012

DOI:

10.3791/3925-v

DOI:
10.3791/3925-v

•

10:42 min
October 22, 2012

•

4 Views
, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology,Roswell Park Cancer Institute, 3Department of Medicine,University at Buffalo School of Medicine

Summary

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NADPH-Oxidase ist die wichtigste Quelle von reaktiven Sauerstoffspezies (ROS) in Phagozyten. Aufgrund der Kurzlebigkeit von ROS, ist es schwierig zu messen und zu überwachen ROS Ebenen in lebenden Tieren. Ein minimal invasives Verfahren zur seriellen Quantifizierung von ROS in lebenden Mäusen beschrieben.

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Cite this Article

Han, W., Li, H., Segal, B. H., Blackwell, T. S. Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models. J. Vis. Exp. (68), e3925, doi:10.3791/3925 (2012).

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