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人力蕈状味觉​​乳头细胞的分离和培养
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Human Fungiform Taste Papillae Cells
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人力蕈状味觉​​乳头细胞的分离和培养

DOI:
10.3791/3730-v

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08:46 min

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May 17, 2012

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, ,
1Monell Chemical Senses Center, 2New York University College of Dentistry, 3AFB International

Chapters

  • 00:05Title
  • 01:29Human Fungiform Taste Papillae Digestion
  • 02:59Culturing of Human Fungiform Taste Papillae Cells
  • 05:36Freezing and Thawing Cultured Human Fungiform Taste Papillae Cells
  • 07:09Results: Isolation and Culture of Human Fungiform Taste Papillae Cells
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

我们旨在建立一个孤立和维护人类菌状的味道乳头细胞的长期文化重现协议。活检取自人体的菌状乳头细胞成功地保持了八个多通道（12个月）没有丧失活力的文化。

Tags

IsolationCultureHumanFungiform Taste Papillae CellsTaste CellsHistologicalMolecularPhysiological CharacteristicsDetectionSimple StimuliComplex Chemical MoleculesFoodsTaste BudsProtocolCulturingMultiple Cell CyclesMolecular PropertiesRegenerative PropertiesFunctional PropertiesSensory CellsPrimary CultureExplant CulturesRodentsSemi-intact Taste BudsLong-term CulturesViabilityTaste Cell ProliferationDifferentiation

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