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Isolamento e Cultura de Células Papila fungiforme Humanos Taste
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Human Fungiform Taste Papillae Cells

Isolamento e Cultura de Células Papila fungiforme Humanos Taste

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14,194 Views

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08:46 min

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May 17, 2012

DOI:

10.3791/3730-v

DOI:
10.3791/3730-v

•

08:46 min
May 17, 2012

•

3 Views
, ,
1Monell Chemical Senses Center, 2New York University College of Dentistry, 3AFB International

Summary

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Nosso objetivo foi desenvolver um protocolo reprodutível para isolar e manter a longo prazo culturas de células humanas fungiformes papilas gustativas. As células de humano papilas fungiformes obtidos por biópsia com êxito foram mantidas em cultura durante mais de oito passagens (12 meses) sem perda de viabilidade.

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Cite this Article

Ozdener, H., Spielman, A. I., Rawson, N. E. Isolation and Culture of Human Fungiform Taste Papillae Cells. J. Vis. Exp. (63), e3730, doi:10.3791/3730 (2012).

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