Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Isolation and Culture of Human Fungiform Taste Papillae Cells
Kapitel
- 00:05Titel
- 01:29Human Fungiform Taste Papillae Digestion
- 02:59Culturing of Human Fungiform Taste Papillae Cells
- 05:36Freezing and Thawing Cultured Human Fungiform Taste Papillae Cells
- 07:09Results: Isolation and Culture of Human Fungiform Taste Papillae Cells
- 08:05Conclusion
We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.
Tags
IsolationCultureHumanFungiform Taste Papillae CellsTaste CellsHistologicalMolecularPhysiological CharacteristicsDetectionSimple StimuliComplex Chemical MoleculesFoodsTaste BudsProtocolCulturingMultiple Cell CyclesMolecular PropertiesRegenerative PropertiesFunctional PropertiesSensory CellsPrimary CultureExplant CulturesRodentsSemi-intact Taste BudsLong-term CulturesViabilityTaste Cell ProliferationDifferentiation
