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Isolation and Culture of Human Fungiform Taste Papillae Cells
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May 17, 2012
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We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.
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IsolationCultureHumanFungiform Taste Papillae CellsTaste CellsHistologicalMolecularPhysiological CharacteristicsDetectionSimple StimuliComplex Chemical MoleculesFoodsTaste BudsProtocolCulturingMultiple Cell CyclesMolecular PropertiesRegenerative PropertiesFunctional PropertiesSensory CellsPrimary CultureExplant CulturesRodentsSemi-intact Taste BudsLong-term CulturesViabilityTaste Cell ProliferationDifferentiation
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Ozdener, H., Spielman, A. I., Rawson, N. E. Isolation and Culture of Human Fungiform Taste Papillae Cells. J. Vis. Exp. (63), e3730, doi:10.3791/3730 (2012).
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