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剪切载荷:一个有用的工具,用于检查视网膜神经细胞之间的缝隙连接示踪剂耦合程度
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
Please note that all translations are automatically generated.
Click here for the English version.
剪切载荷:一个有用的工具,用于检查视网膜神经细胞之间的缝隙连接示踪剂耦合程度
DOI:
10.3791/3180-v
•
10:11 min
•
January 12, 2012
•
Hee Joo Choi
,
Christophe P. Ribelayga
,
Stuart C. Mangel
1
Department of Neuroscience
,
Ohio State University College of Medicine
,
2
Department of Ophthalmology and Visual Science
,
University of Texas Medical School
Chapters
00:05
Title
01:31
Intact Neuron Retinal Preparation
02:38
Cut-loading
05:59
Quantification of Tracer Coupling Using ImageJ
08:22
Representative Results for Photoreceptor Cell Tracer Coupling Determined by Cut-loading Technique
09:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述一个简单方便的方法,以确定视网膜神经细胞之间的缝隙连接示踪剂耦合程度。这种技术,使一个完整的视网膜中的神经元之间的电突触的功能,不同的光照条件下,在白天和晚上不同时间的调查。
Tags
Gap Junction Tracer Coupling
Retinal Neurons
Electrical Synaptic Transmission
Gap Junctions
Electrical Communication
Modulated
Gap Junction Channels
Intracellular Signaling Molecules
Small Metabolites
Metabolic Communication
Chemical Communication
Neurotransmitters
Coupled Cells
Tracer Molecules
Iontophoretic Injection
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