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Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
Chapters
- 00:05Title
- 01:31Intact Neuron Retinal Preparation
- 02:38Cut-loading
- 05:59Quantification of Tracer Coupling Using ImageJ
- 08:22Representative Results for Photoreceptor Cell Tracer Coupling Determined by Cut-loading Technique
- 09:35Conclusion
An easy and convenient method to determine the extent of gap junction tracer coupling between retinal neurons is described. This technique enables one to investigate the function of the electrical synapses between neurons in the intact retina under different illumination conditions and at different times of the day and night.
Tags
Gap Junction Tracer CouplingRetinal NeuronsElectrical Synaptic TransmissionGap JunctionsElectrical CommunicationModulatedGap Junction ChannelsIntracellular Signaling MoleculesSmall MetabolitesMetabolic CommunicationChemical CommunicationNeurotransmittersCoupled CellsTracer MoleculesIontophoretic Injection
