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September 26, 2012
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A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.
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Douglas, J. N., Gardner, L. A., Lee, S., Shin, Y., Groover, C. J., Levin, M. C. Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis. J. Vis. Exp. (67), e4154, doi:10.3791/4154 (2012).
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