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Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
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Neuroscience
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JoVE Journal Neuroscience
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

DOI:
10.3791/50701-v

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07:08 min

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July 31, 2013

•

,
1NRW Research Group ‘Dendritic integration in the CNS’, Department of Epileptology,University of Bonn, 2Neuronal Networks Group,Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE)

Chapters

  • 00:05Title
  • 01:01Prepare and Test the Iontophoresis Pipettes
  • 02:38Generate a Postsynaptic Iontophoretic Potential
  • 04:56Representative Results for Fast Micro-iontophoresis of Glutamate and GABA
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

In this article we introduce fast micro-iontophoresis of neurotransmitters as a technique to investigate integration of postsynaptic signals with high spatial and temporal precision.

Tags

Micro-iontophoresisGlutamateGABASynaptic IntegrationDendritic TreeNeuronal OutputExcitatory InputsInhibitory InputsSpatial IntegrationTemporal IntegrationTwo-photon Scanning MicroscopeNeurotransmitter ReleasePresynapticPostsynaptic

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