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April 19, 2012
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A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.
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Single-cell ProfilingDeveloping Retinal NeuronsMature Retinal NeuronsCell-type Specific MarkersGene Expression ProgramsIndividual CellsTransient Gene Expression ProgramsCellular DiversityCentral Nervous SystemDistinct Cell TypesComplex Neural CircuitsRare Neuronal TypesMolecular ClassificationAnalysis Of Cell TypesNervous SystemMechanisms Of Neural Development
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Goetz, J. J., Trimarchi, J. M. Single-cell Profiling of Developing and Mature Retinal Neurons. J. Vis. Exp. (62), e3824, doi:10.3791/3824 (2012).
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