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Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

15,195 Views

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10:11 min

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January 12, 2012

DOI:

10.3791/3180-v

DOI:
10.3791/3180-v

•

10:11 min
January 12, 2012

•

6 Views
, ,
1Department of Neuroscience,Ohio State University College of Medicine, 2Department of Ophthalmology and Visual Science,University of Texas Medical School

Summary

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An easy and convenient method to determine the extent of gap junction tracer coupling between retinal neurons is described. This technique enables one to investigate the function of the electrical synapses between neurons in the intact retina under different illumination conditions and at different times of the day and night.

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Choi, H. J., Ribelayga, C. P., Mangel, S. C. Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons. J. Vis. Exp. (59), e3180, doi:10.3791/3180 (2012).

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