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Neuroscience
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测绘激光扫描Photostimulation抑制神经回路
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
Please note that all translations are automatically generated.
Click here for the English version.
测绘激光扫描Photostimulation抑制神经回路
DOI:
10.3791/3109-v
•
09:50 min
•
October 06, 2011
•
Taruna Ikrar
,
Nicholas D. Olivas
,
Yulin Shi
,
Xiangmin Xu
2
1
Department of Anatomy and Neurobiology, School of Medicine
,
University of California, Irvine
,
2
Department of Biomedical Engineering, School of Engineering
,
University of California, Irvine
Chapters
00:05
Title
01:32
Brain Slice Preparation
02:17
Equipment Startup
03:24
Whole Cell Patch-clamp Recording
05:52
Laser Scanning Photostimulation
07:44
Representative Results for Electrophysiological Recording and Photostimulation Mapping
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了激光扫描photostimulation在表达绿色荧光蛋白在有限的抑制性神经元的人口的转基因小鼠的全细胞记录相结合的方法。该技术可以广泛的测绘和定量分析当地具体的抑制皮层神经元的突触电路。
Tags
Inhibitory Neurons
Neuronal Circuits
Laser Scanning Photostimulation
Cortical Function
Subtypes
Immunochemical Properties
Morphological Properties
Physiological Properties
Local Circuit Connections
Excitatory Synaptic Input
Inhibitory Synaptic Input
Laser Scanning Photostimulation (LSPS)
Electrical Stimulation
Glutamate Puff Stimulation
Mapping Resolution
Cortical Circuit Analysis
Whole-cell Patch Clamping
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