A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,722 Views
•
06:40 min
•
November 25, 2015
DOI:
Summary
Automatically generated
We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.
Read Article
Cite this Article
Albrecht, O., Klug, A. Laser-guided Neuronal Tracing In Brain Explants. J. Vis. Exp. (105), e53333, doi:10.3791/53333 (2015).
Copy