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Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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October 06, 2011
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This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.
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Inhibitory NeuronsNeuronal CircuitsLaser Scanning PhotostimulationCortical FunctionSubtypesImmunochemical PropertiesMorphological PropertiesPhysiological PropertiesLocal Circuit ConnectionsExcitatory Synaptic InputInhibitory Synaptic InputLaser Scanning Photostimulation (LSPS)Electrical StimulationGlutamate Puff StimulationMapping ResolutionCortical Circuit AnalysisWhole-cell Patch Clamping
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Ikrar, T., Olivas, N. D., Shi, Y., Xu, X. Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation. J. Vis. Exp. (56), e3109, doi:10.3791/3109 (2011).
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