Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
17,126 Views
•
09:50 min
•
October 06, 2011
DOI:
Özet
Automatically generated
This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.
Explore More Videos
Inhibitory NeuronsNeuronal CircuitsLaser Scanning PhotostimulationCortical FunctionSubtypesImmunochemical PropertiesMorphological PropertiesPhysiological PropertiesLocal Circuit ConnectionsExcitatory Synaptic InputInhibitory Synaptic InputLaser Scanning Photostimulation (LSPS)Electrical StimulationGlutamate Puff StimulationMapping ResolutionCortical Circuit AnalysisWhole-cell Patch Clamping
Read Article
Cite this Article
Ikrar, T., Olivas, N. D., Shi, Y., Xu, X. Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation. J. Vis. Exp. (56), e3109, doi:10.3791/3109 (2011).
Copy