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Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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神经科学
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JoVE 杂志 神经科学
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

17,126 Views

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09:50 min

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October 06, 2011

DOI:

10.3791/3109-v

DOI:
10.3791/3109-v

•

09:50 min
October 06, 2011

•

3 Views
, , ,
1Department of Anatomy and Neurobiology, School of Medicine,University of California, Irvine, 2Department of Biomedical Engineering, School of Engineering,University of California, Irvine

Summary

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This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.

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Ikrar, T., Olivas, N. D., Shi, Y., Xu, X. Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation. J. Vis. Exp. (56), e3109, doi:10.3791/3109 (2011).

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