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Observation directe de la phagocytose et NET-formation par les neutrophiles dans les poumons infectés en utilisant deux microscopie biphotonique
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
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Observation directe de la phagocytose et NET-formation par les neutrophiles dans les poumons infectés en utilisant deux microscopie biphotonique

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20,463 Views

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08:50 min

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June 02, 2011

DOI:

10.3791/2659-v

DOI:
10.3791/2659-v

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08:50 min
June 02, 2011

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2 Views
, , , ,
1Institute for Molecular and Clinical Immunology,Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation,Helmholtz Center for Infection Research

Summary

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Nous montrons, comment utiliser les deux photons de microscopie pour l'observation de la dynamique des granulocytes neutrophiles dans les poumons infectés alors qu'ils phagocyter des pathogènes ou de produire des neutrophiles pièges extracellulaires (NET).

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Cite this Article

Hasenberg, M., Köhler, A., Bonifatius, S., Jeron, A., Gunzer, M. Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy. J. Vis. Exp. (52), e2659, doi:10.3791/2659 (2011).

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