We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).
Hasenberg, M., Köhler, A., Bonifatius, S., Jeron, A., Gunzer, M. Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy. J. Vis. Exp. (52), e2659, doi:10.3791/2659 (2011).