Here we describe methods using spinning disk confocal microscopy to image single phagocytic events by mouse resident peritoneal macrophages. The protocols can be extended to other phagocytic cells.
Horsthemke, M., Wilden, J., Bachg, A. C., Hanley, P. J. Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages. J. Vis. Exp. (140), e57566, doi:10.3791/57566 (2018).