Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Mike Hasenberg; Anja K&#246;hler; Susanne Bonifatius; Andreas Jeron; Matthias Gunzer

doi:10.3791/2659

Journal

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Imunologia e Infecção

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Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

20,469 Views

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08:50 min

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June 02, 2011

DOI:

10.3791/2659-v

DOI:

10.3791/2659-v

•

08:50 min

June 02, 2011

•

2 Views

Mike Hasenberg¹, Anja Köhler¹, Susanne Bonifatius¹, Andreas Jeron², Matthias Gunzer¹

¹Institute for Molecular and Clinical Immunology,Otto-von-Guericke University Magdeburg, ²Department of Immunoregulation,Helmholtz Center for Infection Research

Summary

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

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Phagocytosis NET-formation Neutrophils Infected Lungs 2-photon Microscopy Gas Exchange Defense Strategies Humoral Immune Mechanisms Cellular Immune Mechanisms Recruitment Of Neutrophils Cytotoxic Chemicals Extracellular DNA-NETs Bacteria Fungi Lung Infection Viable Lung Slices Intratracheal Infection Aspergillus Fumigatus Multicolor Time-lapse Microscopy

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Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

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