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Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
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June 02, 2011
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We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).
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PhagocytosisNET-formationNeutrophilsInfected Lungs2-photon MicroscopyGas ExchangeDefense StrategiesHumoral Immune MechanismsCellular Immune MechanismsRecruitment Of NeutrophilsCytotoxic ChemicalsExtracellular DNA-NETsBacteriaFungiLung InfectionViable Lung SlicesIntratracheal InfectionAspergillus FumigatusMulticolor Time-lapse Microscopy
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Hasenberg, M., Köhler, A., Bonifatius, S., Jeron, A., Gunzer, M. Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy. J. Vis. Exp. (52), e2659, doi:10.3791/2659 (2011).
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