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Competitiva Ensaios Homing para Estudar Gut trópico-Migração de Células T
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Competitive Homing Assays to Study Gut-tropic T Cell Migration
DOI:

10:25 min

March 01, 2011

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Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:59Ex vivo Generation of Gut-Homing and Control T cells
  • 04:25Analysis of Gut-Homing Receptors on Activated T cells
  • 05:21T cell Labeling with CFSE and CMTMR Cell Trackers
  • 07:42Analysis of T cell Homing
  • 08:58RA-induced Expression of a4B7 and CCR9 Direct T Cell Migration to the Gut
  • 09:50Conclusion

Summary

Automatic Translation

Competitiva homing experimentos permitem avaliar diretamente as propriedades migratória de duas populações celulares diferentes em um único rato. Aqui nós ilustrar este procedimento através da comparação da migração de ex-vivo gerado gut trópico-versus non-gut células T trópico.

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